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Objective:To discuss the diagnostic methods, clinical features and treatment options of bronchogenic cysts.Methods:A total of 86 patients with bronchogenic cysts and 5 patients with esophageal cysts and esophageal cysts were selected from January 2011 to May 2020 in the Affiliated Tumor Hospital of Harbin Medical University. There were 37 males and 49 females with bronchogenic cysts, aged 23 to 70(49.27±10.70)years old. According to the location of the disease, the patients were divided into mediastinal type(65 cases, 75.6%); intrapulmonary type(21 cases, 24.4%); bronchogenic cyst originating from the esophagus(9 cases, 10.5%).Results:The preoperative diagnosis coincidence rate was 9.3% in 8 cases. The rate of thoracoscopic surgery(59.3% in 51 cases), compared with the indwelling time of thoracic drainage tube after thoracotomy[(3.80±1.25) days vs.(4.97±1.54)days, P<0.001] and hospital stay[(7.08±1.75) days vs.(9.60±2.58)days, P<0.001] significantly shortened. 65 cases(71.4%, 65/91) were successfully followed up, with a median follow-up time of 34(2-111) months, and no recurrence was found. Conclusion:Bronchial cysts have no characteristic clinical manifestations, and it is difficult to make a clear diagnosis before surgery. Chest MRI has a great advantage in the diagnosis of cysts. For most cases, thoracoscopic surgery can achieve better clinical treatment results and has minimally invasive advantages. It is difficult to distinguish between bronchogenic cysts that originated in the esophagus and esophageal cysts, and there is no significant difference in clinical characteristics.
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Objective To investigate the correlation between serum sFas and sLOX-1 with occurrence and development of acute coronary syndrome (ACS).Methods A total of 52 patients definitely diagnosed ACS (ACS group) by coronary artery angiography (CAG) were enrolled,including 23 cases of unstable angina (UA group) and 29 cases of acute myocardial infarction (AMI group),and contemporaneous 58 cases of non-coronary arterial stenosis confirmed by CAG were selected as the control group (NC group).The serum levels of sFas and sLOX-1 were measured by enzyme linked immunosorbent assay.Results Compared with the NC group,the serum levels of sFas and sLOX-1 in the ACS group were increased,the serum levels of sFas and sLOX-1 in the AMI group and UA group were higher than those in the NC group,moreover which in the AMI group were higher than those in the UA group (P＜0.01).The serum sFas level in the ACS group was positively correlated with the sLOX-1 level (r=0.825,P=0.001),but both had no obvious correlation with the serum levels of CK-MB and cTnⅠ (P＞0.05).Conclusion High levels of serum sFas and sLOX-1 may be the risk factors of ACS.
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Objective The aim of this study is to evaluate whether video -assisted thoracoscopic surgi-cal( VATS) lobectomy is as effective as open thoracotomy lobectomy for complete dissection of the mediastinal lymph node(MLN).Methods Patients with clinical stage N0 lung cancer who underwent lobectomy between January 2008 and June 2013were retrospectively evaluated based on the LN station resected and lobectomy proce -dure used,and a resection ratio was calculated .Nodal stage and the proportion of patients ,from whom at least three MLNs and station 7were dissected and compared by lobectomy type .Results Of the 201 patients enrolled in the study,84 and 117 underwent VATS and open thoracotomy lobectomies ,respectively.The mean number of LNs dissected at station 3a was similar in the two groups (1.34 ±2.58 vs.1.52 ±1.78;P>0.05),but the re-section ratio differed(39%sv .63%;P 0.05).There were no differences in the number of LNs dissected or resection ratio between the two groups for stations 4 L,5 L6, L,7 L, and9 L for the left-sided approaches .Only station 8L showed significant differences between the VATS and open thoracotomy groups in the number of LNs dissected (0.12 ±0.44 vs.0.46 ±0.71;P0.05). The Kaplan-Meier 5-year survival was also similar between the two groups (log-rank test,P>0.05).Con-clusion VATS lobectomy is as effective as open thoracotomy lobectomy for the dissection of MLNs .Thus,VATS lobotomy resection will continue to be offered as the best choice for patients with clinical stage N0.
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Objective Two-incision video-assisted thoracic surger relieved post operative pain when compared with open thoractomy ,while it is rarely reported worldwide ,most thoracic surgeons think it is hard to finish the complicated operation and it is not safe .We compared the safety between open and two -incision VATS.Methods Bwteen Febrary 2009 to December 2011 ,a total of 334 cases with clinical early -staged lung cancer of open thoracotomy were performed ,66 cases were completely performed with 2-incision VATS,17 cases were transferred to open thoracotomy defined as two -incision VATS assisted thoracotomy .We compared and ana-lyzed open thoracotomy with two -incision VATS in operating time ,and pre,post and total period of hospitaliza-tion,postoperative chest tube removal time ,postoperative complications .Results Operating time in the left lower lobe of both traditional open thoracotomy and two -incision VATS was 162.5 ±6.5 and 185.8 ±12.8 minutes re-spectively(P=0.1228),there was no statistical significance for the remaining parts of the lobectomy ,the operat-ing time of open thoracotomy was shorter than two -incision VATS.The overall complication and perioperative mortality rate of open thoracotomy and two -incision VATS were 10.2% and 15.0%(P=0.238),and 2.0%and 0.0%(P=1.000)respectively,there was no statistical significance.Conclusion The lobectomy and lymph node dissections for 2-incision VATS in treating clinical stage I lung cancer is feasible and safe .
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Objective To study the change of endothelial function during myocardial ischemia-reperfusion injury in rabbits and the effect of Rasuvastatin. Method Sixteen New Zealand rabbits were randomLy divided into two groups: ischemia/reporfusian injury group (control group) and Resuvastatin group(drug group). The myocar-dial ischemia-reperfusion model was established by occlusion of left anterior descending coronary artery for 40 min-utes evidenced by the elevation of the ST segment≥0.2 my on ECG waveform, and after release of ooclusion, the ST segment of ECG retttmed to 1/2 or more of the normal wavefonn, which was the evidence of successful reperfu-sion. The rabbit serum nitric oxide (NO) and plasma endothelia-1 (ET-1) content were assayed before occlusion, 40 minutes, 60 minutes and 180 minutes after reperfusion. SPSS11.5 software was used for ANOVA(Repested Measurement designs). P<0.05 was considered as statistically significant. Results There were no significant differences in serum NO and plasma ET-1 between two groups bsfore isehemia, but 40 minutes, 60 minutes and 180 minutes after reperfusion, the levels of serum NO in drug group were higher than those in control group [(82.000±13.825), (63.375±17.541), (50.250±18.987)μmol/L vs. (63.125±18.962), (43.500± 16.518), (29.625±14.162) μmol/L, P<0.05], and the levels of plasma ET-1 content in drug group were lower than those in the control group [(282.541±38.928), (315.152±55.263), (377.795±60.427) pg/mL vs. (331.785±35.341),(375.914±5.204),(459.829±70.110) pg/mL, P<0.05]. Conclusions By the means of increasing serum NO and decreasing plasma ET-1, rosuvastatin can improve the endothelial function in rabbits with isehemia-reperfusion injury.
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<p><b>BACKGROUND</b>Biochemical and genetic researches suggest that ras protein plays an important role in transduction process of cell proliferation differentiation signals from activated transmembrane receptors to substream protein kinases. This study is to explore the expression of ras, p38, both of which are members of MAPK (mitogen activated protein kinases) signal transduction pathway, and STAT3 (signal transducer and activator of transcription 3) in non-small cell lung cancer, and the association among them.</p><p><b>METHODS</b>Forty-two resected lung cancer and paracancerous lung tissue samples were used to determine the protein expression of ras, p38 and STAT3 with Western blot, the mRNA expression of p38 and STAT3 in lung cancer tissues of various ras protein expression with RT-PCR. The location of p38 and STAT3 in lung cancer tissues was revealed with immunofluorescent staining.</p><p><b>RESULTS</b>The relative protein expressions of ras, p38 and STAT3 were 0.6012, 0.6724, 0.5119 in cancer tissues, and 0.2793, 0.3071, 0.1917 in paracancerous lung tissues, respectively (P < 0.01). The protein and mRNA expressions of p38 and STAT3 ( 0.7624 and 0.6262; 1.0309 and 1.0538) in cancer tissues with higher ras protein expression were remarkably higher than those with lower ras protein expression (0.4715 and 0.2569; 0. 6569 and 0.3437, P < 0.01). There was a significant correlation between the expression of ras, p38 and STAT3 (correlation coefficient: 0.809 and 0.842, P < 0.01), and so was the p38 and STAT3 (correlation coefficient: 0.829, P < 0.01).</p><p><b>CONCLUSIONS</b>Abnormal expressions of STAT3 and some factors of ras-MAPK signal transduction pathway exist in the oncogenesis and development of non-small cell lung cancer, and many of them may have crosstalk.</p>
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<p><b>BACKGROUND</b>The roles of nuclear factor kappa B (NF-kappa B) and activating protein 1 (AP-1) become well-known in cell apoptosis and proliferation and oncogenesis. This study aims to explore expression of NF-kappa B and AP-1 in non-small cell lung cancer (NSCLC), the relationship between them and their function on expression of cyclin D1 and caspase 3 in NSCLC.</p><p><b>METHODS</b>Protein expression of NF-kappa B, AP-1 , cyclin D1 and caspase 3 was detected by Western blot and mRNA expression of cyclin D1 and caspase 3 by RT-PCR. Correlation between NF-kappa B and AP-1 was analyzed using bivariate correlate analysis.</p><p><b>RESULTS</b>Of all 45 NSCLC patients, the expression of NF-kappa B and AP-1 in NSCLC was higher than that in normal lung tissues (0.6047 versus 0.2798, P < 0.01). Protein and mRNA expression of cyclin D1 in lung cancer tissues with higher NF-kappa B and AP-1 protein expression was higher than in those with lower NF-kappa B and AP-1 protein expression (P < 0.01), while protein and mRNA expression of caspase 3 in lung cancer tissues with higher NF-kappa B and AP-1 protein expression was lower than in those with lower NF-kappa B and AP-1 protein expression (P < 0.01). There was significant correlation between NF-kappa B and AP-1 (r=0.800, P < 0.01).</p><p><b>CONCLUSIONS</b>Transcription factors, NF-kappa B and AP-1 may play important roles in oncogenesis and development of NSCLC.</p>